Nano Workshop

Poster # 1

Application of silver-based biocides in face masks intended for general use requires regulatory control

Presenter: Jan Masta (Sciensano)

Abstract: Silver-based biocides are applied in face masks because of their antimicrobial properties. The added value of biocidal silver treatment of face masks to control SARS-CoV-2 infection needs to be balanced against possible toxicity due to inhalation exposure. Direct measurement of silver (particle) release to estimate exposure is problematic. Therefore, this study optimized methodologies to characterize silver-based biocides directly in the face masks, by measuring their total silver content using ICP-MS and ICP-OES bas   read more...

Poster # 2

Comparison of biodistribution of cerium oxide nanoparticles after repeated oral administration by gavage or snack in Sprague Dawley rats

Presenter: Alicja Mortensen (The National Research Centre for the Working Environment)

Abstract: The rate of translocation of ingested nanoparticles (NPs) and how the uptake is affected by a food matrix are key aspects of health risk assessment. In this study, female Sprague Dawley rats (N=4/group) received 0, 1.4, or 13 mg of cerium oxide (CeO2 NM-212) NPs/rat/day by gavage or in a chocolate spread snack 5 days/week for 1 or 2 weeks followed by 2 weeks of recovery. A dose and time-dependent uptake in the liver and spleen of 0.1–0.3 and 0.004–0.005 parts per million (ng/mg) of the total administered dose was f   read more...

Poster # 3

Analysis of common polymers using hyphenated TGA-FTIR-GCMS towards the development of an analytical database for micro- and nanoplastics identification, characterization, and quantitation

Presenter: Sungyoon Jung (University of Florida)

Abstract: The presence of micro- and nanoplastics in marine and terrestrial ecosystems is causing environmental, ecological, and human exposure concerns, resulting in a recent increase in publications, news articles and reports. Many comprehensive reviews (1-5) and reports (6-10) on this subject identified significant knowledge gaps in analytical methods and methodologies for the isolation, separation, identification and quantification of micro- and nanoplastics mixtures from real-world samples. Publicly available databases fo   read more...

Poster # 4

Occurrence and dietary exposure assessment of heavy metals in baby foods in the Kingdom of Saudi Arabia

Presenter: Najla Alharbi (Saudi Food and Drug Authority)

Abstract: Early childhood exposure to heavy metals like arsenic (As), cadmium (Cd), and lead (Pb) through baby foods unfolds many concerns about their toxic effects on growth and health. In this study, occurrence and dietary intake of As, Cd, and Pb in stage 1 infant formula (0–6 months), stage 2 infant formula (7–12 months), cereal-based meals, and biscuits were estimated. First, the levels of As, Cd, and Pb were determined with ICP-MS, followed by the calculation of estimated daily intake (EDI), target hazard quo- tient (THQ   read more...

Poster # 5

Application of liposomal nanoparticles produced via continuous manufacturing as reference standard materials

Presenter: Diane Burgess (University of Connecticut)

Abstract: Introduction: Following the recent success of lipid nanoparticles (LNPs) for the delivery of mRNA COVID-19 vaccines, there is an anticipated increase in demand for such lipid-based therapeutics and vaccines. Accordingly, there is incentive to develop suitable reference materials. Such materials would provide significant benefits to regulatory agencies and industry for product development, quality control, and technology transfer. Our laboratory has developed a continuous manufacturing platform that can produce mono   read more...

Poster # 6

Heavy Metals And Pesticide Residues In Small Farm Cheese Production In Croatia-Challange Betweeen Quality And Quantity

Presenter: Adela Krivohlavek (Teaching institute of public health dr. Andrija Štampar)

Abstract: In the last few decades, with the global health awareness on the rise, increased interest in health effects associated with cheese consumption has been recognised. Recently, studies have emphasized cheese as the source of bioactive compounds demonstrating antioxidant, antimicrobial, anti-inflammatory, immunomodulatory and analgesic activity. The aim of this article was to analyse pesticide residues and heavy metal content cheeses produced in Croatia. Sample consisted of 80 cheses of different ruminant origin (sheep,   read more...

Poster # 7

Regulatory challenges for novel ways to defeat post-weaning diarrhoea in pigs

Presenter: Klaus Hellmann (Klifovet GmbH, part of the Argenta Group)

Abstract: Antimicrobial resistance (AMR) in livestock is a public health threat due to the risk of zoonotic transmission to humans and its negative consequences on animal health and welfare when diseases cannot be treated. Antimicrobial treatment options for post- weaning diarrhoea in pigs are increasingly limited because of planned restrictions in the use of zinc oxide and colistin, two current choices for prevention/treatment of post- weaning diarrhoea caused by enterotoxic E. coli (ETEC). Faecal filtrate treatment (FFT) was   read more...

Poster # 8

Enzyme-Based Methionine Depletion In Cancer Cells: Insight Into Delivery Strategies Of Methionine Gamma-Lyase

Presenter: Samanta Raboni (Department of Food and Drug, University of Parma )

Abstract: The dependence of cancer cells on the essential amino acid methionine has triggered extensive investigations of methionine γ-lyase (MGL) - a bacterial pyridoxal 5’-phosphate-dependent enzyme that degrades methionine - as a therapeutic tool capable of inhibiting cancer cells growth through methionine starvation. Our most recent research on the MGL-mediated depletion of methionine is devoted on the development of a novel delivery platform for MGL - based on enzyme adsorption on spherical citrate-capped gold nanoparti   read more...

Poster # 9

Early awareness and action system for advanced materials (Early4AdMa)

Presenter: Agnes Oomen (RIVM)

Abstract: Here, a systematic approach (‘Early4AdMa’) is proposed to identify and describe potential safety, sustainability and regulatory issues of advanced materials at the early stages of their development or use. The approach described here is based on a system developed by the RIVM (NL), BfR (DE), BAuA (DE) and UBA (DE), and adapted within the OECD WPMN Steering Group on Advanced Materials. The outcome of the approach can inform decision makers, policy makers, risk assessors, and regulators and could facilitate regulatory   read more...

Poster # 10

EFSA NAMs4NANO PROJECT: Integration of New Approach Methodologies (NAMs) results in chemical risk assessments using case studies addressing nanoscale considerations

Presenter: Maria Chiara Astuto (EFSA)

Abstract: Based on the recommendations of the EFSA Roadmap on NAMs, EFSA has funded projects to promote the use of NAM-based IATA to fill data gaps in the risk assessment practice, integrating the available toxicological information with newly generated NAM-based studies developing proof-of-concept case studies. An example in the field of nanomaterials is the EFSA NAMs4NANO Project, established thought partnership with EU Member State organisations. The new EFSA NAMs4NANO Project aims to develop a “Qualification system for NAM   read more...

Poster # 11

From laboratory to harmonisation: OECD TG126 on Hydrophobicity index, a new method for nanomaterials characterisation

Presenter: Andrea Valsesia (European Commission, Joint Research Centre (JRC), Ispra, Italy)

Abstract: The JRC has led the work towards the development and adoption of the OECD TG for VSSA of nanomaterials (TG 124). The main steps undertaken are described. These include : i) preparatory steps with experts for the selection of testing materials, the information exchange on existing protocols and drafting a standard operating procedure (SOP) ii) organisation and evaluation of an interlaboratory study to gain information on the domain applicability and method transferability and iii) drafting and review of the test g   read more...

Poster # 12

First steps towards the qualification of a Human organoid model for intestinal toxicity testing  

Presenter:  Deborah Stanco (European Commission, Joint Research Centre (JRC), Ispra, Italy)

Abstract: Intestinal organoids mimic the structure, function and physiology of human intestine in vitro. Thanks to these advantages, intestinal human organoids (iHO) are suitable for various applications from drug discovery to translational research in biomedical field. These benefits make intestine human organoids (iHO) attractive for a range of applications, including drug discovery and toxicity assessments. There are currently no standards in place to monitor whether iPSCs successfully differentiate into healthy or sick tis   read more...

Poster # 13

TiO2 nanoparticle accumulation in different cell types 

Presenter:  Alessia Bogni (European Commission, Joint Research Centre (JRC), Ispra, Italy)

Abstract: The current understanding on potential hazards associated with titanium dioxide in nanoforms is still not fully conclusive. In the presented study, we investigated the capability of different cellular models to internalise and accumulate TiO2. The use of radioactive labelled TiO2 allowed us to quantify the accumulated nanomaterial in cells even after 30 days in culture. Such in vitro platforms can support the investigation of long-term effects of nanomaterial with biological systems but require an in depth characteri   read more...

Poster # 14

Environmental relevant nanoplastics models for laboratory testing

Presenter: Andrea Varsesia (European Commission, Joint Research Centre (JRC), Ispra, Italy)

Abstract: Reference particle models are essential to obtain an accurate perspective of how environmental nanoplastics behave in natural systems and to generate data on their environmental fate and impact on living organisms. However, although the current available models are particularly important for filling the initial knowledge gaps on nanoplastics, they do not demonstrate enough diversity and/or accuracy to represent the actual heterogeneity of the physical and chemical properties of environmental nanoplastics. In this fr   read more...

Poster # 15

Building of AOPs to support nanomaterial testing in vitro

Presenter: Deborah Stanco (European Commission, Joint Research Centre (JRC), Ispra, Italy)

Abstract: Based on the recommendations of EFSA “Guidance on risk assessment of nanomaterials to be applied in the food and feed chain”, the NAMS4NANO project was launched to establish proof of concept case studies addressing nano specific concerns when using new approach methodologies (NAMs). Notably, there is a need to address nano considerations of possible uptake by cells at the intestinal level and potential adversities following the intracellular uptake using NAMs. Adverse Outcome Pathways (AOPs) is a conceptual framewo   read more...

Poster # 16

JRC Nanobiotechnology Laboratory for Health and Safety in the EU

Presenter:  Birgit Sokull-Kluettgen ( European Commission, Joint Research Centre)

Abstract: Overview about activities of JRC’s Nanobiotechnology Lab, including (a) Fast adaptation to emerging health threats; (b) Response to emerging pollutants, i.e. micro(nano)plastics; (c) Support to EU policies to promote Safe & Sustainable Innovation for advanced materials; (d) Open Access to JRC Infrastructure including training & capacity building.

Poster # 17

Scientific protocol underpinning the development and adoption of a new harmonised OECD TG for VSSA of nanomaterials

Presenter:  Josefa Barrero (European Commission, Joint Research Centre)

Abstract: The JRC has led the work towards the development and adoption of the OECD TG for VSSA of nanomaterials (TG 124). The main steps undertaken are described. These include : i) preparatory steps with experts for the selection of testing materials, the information exchange on existing protocols and drafting a standard operating procedure (SOP) ii) organisation and evaluation of an interlaboratory study to gain information on the domain applicability and method transferability and iii) drafting and review of the test g   read more...

Poster # 18

EFSA Case Studies on NAMs

Presenter:  Irene Cataneo (EFSA)

Abstract: In the last few years, EFSA started dedicated projects on New Approach Methodologies (NAMs) to promote their implementation in regulatory risk assessment. Information from animal studies is frequently available, but often presents data gaps that create uncertainties and inconclusive risk assessment. The main objective of the EFSA NAMs projects is to promote a comprehensive chemical risk assessment to fill data gaps in the context of EFSA’s remit (i.e. food and feed area) while fostering the transition towards a mecha   read more...

Poster # 19

EFSA Cross-cutting Working Group on Nanotechnologies and Nano Guidance documents

Presenter:  Djien Liem (EFSA)

Abstract: In the EU context of regulated food and feed products, when an application concerns a material that meets the definition of engineered nanomaterial set out in Regulation (EU) 2015/2283, the application must follow the EFSA Scientific Committee Guidance on Risk Assessment of Nanomaterials, published in 2018 and updated in 2021. This Guidance covers the application areas within EFSA’s remit, including novel foods, food contact materials, food/feed additives, and pesticides, in the context of human and animal health ris   read more...

AI Workshop

Poster # 20

A Walk in PARC WP Hazard Assessment

Presenter: Philip Marx-Stoelting (Germand Federal Institute for Risk Assessment)

Abstract: Current approaches for the assessment of environmental and human health risks due to exposure to chemical substances have served their purpose reasonably well so far. Nevertheless, the assessment systems in place for different chemical regulations are faced with several challenges, ranging from a growing number of chemicals to changes in the types of chemicals and materials produced. This has triggered awareness of the need for a paradigm shift, notably appearing in the CSS, requiring new concepts for chemical risk a   read more...

Poster # 21

Establishing a methodical strategy for the regulatory surveillance of herbal medicine raw materials

Presenter: Faezeh Vahidi Motlagh (Tehran University of Medical Sciences)

Abstract: There are several stages in the supply chain of a mass manufactured herbal medicine. Provision of the raw material, refinement and processing, receiving FDA approvals, distributing the goods through authorized channels of distribution, and ultimately reaching the consumer, are all important components of such an endeavor. How raw ingredients are supervised and regulated is a crucial aspect of herbal medicine mass production that ensures product efficacy and safety for consumers. Naturally, the Food and Drug Administr   read more...

Poster # 22

A Benchmark Dataset for Evaluating Computational Methods of Detecting Pathogens in Metagenomes

Presenter: Olateju Idowu (University College Dublin)

Abstract: Metagenomic analysis has proven to be an invaluable method for determining the taxonomic breakdown of the microbes in an environment or a host. This has led to an interest in the use of metagenomics in public health for pathogen monitoring. However, most computational tools for metagenomic analysis have the general purpose of classifying sequence reads into different taxa, rather than the specific goal of identifying particular pathogens, and distinguishing them from closely or more distantly related taxa. We have de   read more...

Poster # 23

Alternatives to animal experiments in research and regulatory testing: The need to address dilemmas to inform policy

Presenter: David Mawufemor Azilagbetor (Institute for Biomedical Ethics, University of Basel, Switzerland)

Abstract: Several states are putting drastic policies in place to phase out animal use in biomedical research and regulatory testing. As understood from arguments for and against animal research, these political and regulatory moves result from two major unresolved issues: one, the ethical aspects of experimenting on nonhuman animals and second, the robustness of animal research as a methodology of scientific investigation of human physiology. Whilst these issues remain, the contribution of research on animals to human and ve   read more...

Poster # 24

Assessing the safety of food chemicals without animal testing: A NGRA approach 

Presenter: Danilo Basili (Institute of Food Safety and Analytical Sciences, Nestlé, Switzerland )

Abstract: Chemical risk assessment is currently undergoing a paradigm shift driven by ethical considerations, regulatory action and the need to ensure the safety of chemicals using efficient, cost-effective and robust methods. Non-animal approaches represent a powerful alternative to improve safety assessments by using more human-relevant tools providing a good coverage of key biological targets. Next-generation risk assessment (NGRA) provides a framework integrating data coming from diverse so called new approach methodologie   read more...

Poster #25

Using Artificial Intelligence Technology to Solve the Challenges in Microbial Risk Assessment

——A cross-contamination risk assessment model with improved coefficient optimization for Campylobacter

Presenter: Yeru Wang (China national center for food safety risk assessment)

Abstract: Objective: Evaluating the impact of microbial pollution through artificial intelligence methods. To build a more feasible Campylobacter cross-contamination model for poultry slaughter in China. To proposed corresponding suggestions for improving the slaughter and process operations. Highlights: An estimation model for the key coefficient of cross- contamination during the slicing process of broilers was established using artificial intelligence methods. This model uses genetic algorithm(GA) to search for la   read more...

Poster #26

Revision of FSCJ Guidance on Benchmark Dose (BMD) Approach - Introduction of Bayesian Estimation

Presenter: Norimasa Tamehiro (Food Safety Commission Secretariat of Japan)

Abstract: Purpose: The Food Safety Commission of Japan (FSCJ) is a risk assessment organization for science-based assessment of food safety risks to human health in the Cabinet Office of Japanese Government. In 2019, FSCJ published “the Guidance on the Use of the Benchmark Dose (BMD) Approach in Risk Assessment by FSCJ (FSCJ BMD Guidance (”, which provides basic stance and procedures for the use of the BMD approach for deriving a suitab   read more...

GSRS23 Conference

Poster # 27

Pesticide Residue Intake Model (PRIMo) revision 4

Presenter: Violetta Costanzo (TRASYS Greece)

Abstract: The EFSA Pesticide Residue Intake Model (PRIMo) has, since its first version in 2007, become the standard tool used at the EU level to estimate the acute and chronic dietary exposure to pesticide residues. The PRIMo is a deterministic model, primarily used as a prospective screening tool for dietary risk assessment in the framework of Regulation (EC) No 396/2005 and Regulation (EU) No 1107/2009. The aim of the project was to develop a new revision of the tool and transform the former Excel-based calculation spreadshe   read more...

Poster # 28

Evaluating Teratogenic Potential across Compound Categories: Insights from the Mouse Embryoid Body Test Model and Identification of Predictive Gene Markers

Presenter: Yixian Quah (Korea Institute of Toxicology)

Abstract: In vitro tests, like embryonic stem (ES) cell-based assays, provide advantages for teratology research, such as speed, cost-effectiveness, scalability, and mechanistic investigations. To enhance their regulatory application in teratogenic chemical exposure assessment, we aimed to define the applicability domain of the mouse embryoid body test (EBT) model. We cultivated mouse embryonic stem cells (mESCs) in a feeder-free environment, successfully forming embryoid bodies (EBs) using the 384-ultra low attachment (ULA) w   read more...

Poster # 29

Characterisation of human variability in toxicodynamics – towards the development of quantitative Adverse Outcome Pathways (qAOPs)

Presenter: Martijn J. Moné (Leiden Academic Centre for Drug Research (LACDR), Leiden University, The Netherlands)

Abstract: Currently, risk assessment is largely based on animal experimentation. These studies do not reflect human biology or involve mechanistic toxicodynamic information. However, accurate risk assessment using human cells is hampered by limitations in the understanding of the population variability in toxicodynamics. The TD-TRAQ project aims to understand population variability of critical toxicity-related pathways to derive uncertainty factors (UFs) for toxicodynamics. We seek to prove that systematic transcriptomics-base   read more...

Poster # 30

GSRS Conference

Presenter: Justin Wiencek

The Global Coalition for Regulatory Science Research (GCRSR) was established in 2013 under the leadership of the US-FDA. The mission of GCRSR is to foster the uptake of emerging technologies by engaging regulatory agencies in the global context. This is an international coalition with the objectives of facilitating education, scientific training and scientific exchanges in the field of regulatory science. It focuses on research to support regulatory decision making by identifying and promoting best practices to understand a   read more...


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